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Plasmid#120873PurposeExpresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.DepositorInsertCas12c2
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymYPET-CaURA3
Plasmid#118460PurposeYeast tagging vector to create a gene fusion with ymYPET with CaUra3 selectionDepositorInsertymYPET
UseTagsExpressionYeastMutationYPET A206K, F208S, E232L, N235DPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag
Plasmid#109030PurposeWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-ELP[V-30]
Plasmid#67014PurposeExpresses ELP[V-30] in E. coliDepositorInsertELP[V-30]
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo2
Plasmid#25102DepositorInsertpro-SUMO2 (SUMO2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(V266E)
Plasmid#90089PurposeExpresses caspase 3 V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationV266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspWYL1
Plasmid#108306PurposeExpresses E. coli codon optimized RspWYL1 in the pET28a backbone.DepositorInsertRspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only