Addgene

We narrowed to 4,647 results for: IRES

Showing: 1801 - 1820 of 4647 results

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pMXS-IRES-BLAST flag-CAD delta Loop1

Plasmid

#169831

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids F1366 - P1376; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop2

Plasmid

#169832

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids R1398 - S1407; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1406A

Plasmid

#169823

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

Plasmid

#207171

Purpose

expression plasmid for Gal4DBD-HIF-CAD.myc expression

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD Myc tag linker

Use

Tags

2xMyc tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD R1403A R1404A

Plasmid

#188135

Purpose

Expresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD E1367A E1368A

Plasmid

#188143

Purpose

Expresses C-terminal flag-tagged human CAD E1367A E1368A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD P1375A P1376A

Plasmid

#188146

Purpose

Expresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

Plasmid

#191365

Purpose

Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPbaCas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

Plasmid

#191364

Purpose

Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba5Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

Plasmid

#191363

Purpose

Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba4Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

Plasmid

#191366

Purpose

Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm6Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS

Plasmid

#191359

Purpose

Overexpression of NLS-dMisCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dMisCas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

Plasmid

#190079

Purpose

express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

Plasmid

#185717

Purpose

AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Changed Ala 11 to Pro, Val 70 to Pro

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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