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Plasmid#198850PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND1-W86-L14 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro
Plasmid#198858PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND5-W68-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro
Plasmid#198846PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX3-W16-L17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro
Plasmid#198840PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-L17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro
Plasmid#198854PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertND3-Q26-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro
Plasmid#198838PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP6-W68-L16 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro
Plasmid#198856PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND4-W24-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro
Plasmid#198844PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro
Plasmid#198852PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND2-W30-L16 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro
Plasmid#198848PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCYTB-W77-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro
Plasmid#198842PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX1-W25-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo
Plasmid#192339PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLSDepositorInsertmCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS
Plasmid#192317PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLSDepositorInsertmCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe NGF-Erbb2-2xFKBP-HA hygro
Plasmid#192291PurposeRetroviral expression vector for NGF-Erbb2-2xFKBP-HA constructDepositorInsertNGF-Erbb2-2xFKBP-HA (Erbb2 Rat)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro
Plasmid#198860PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND6-W82-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP
Plasmid#194016PurposeExpresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFPDepositorInsertssgRNA(Oxtr.2)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromotercmb and u6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-μ3A-(HA)3
Plasmid#198179PurposeExpression of HA-tagged AP-3 μ3A in mammalian cellsDepositorInsertAP-3 μ3A
UseTagstriple HA tagExpressionMammalianMutationsilent substitution in codon 225PromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_gRNA_3418711_KCNB2
Plasmid#197558PurposegRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)DepositorInsertupstream KCNB2 promoter gRNA (Kcnb2 Rat)
UseGrna with puromycin selectionTagspuromycin resistance cassette under CMV promotionExpressionMammalianMutationPromoterU6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCav3.1/pcDNA3-HE3
Plasmid#197324PurposePlasmid expressing an antigen targeting the Cav3.1 calcium channel subunitDepositorInsertCav3.1 (Cacna1g Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only