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Showing: 1801 - 1820 of 18491 results

  3. pAAV-hSyn-eNpHR 3.0-EYFP

    Plasmid
    #26972
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV5
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Trafficking Signal (TS) ER Export Signal
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26972-plasmid-map-sequence-id-196842

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  4. 7M8

    Plasmid
    #64839
    Purpose
    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.
    Depositor
    David Schaffer, John Flannery
    Article
    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra
    Insert
    7M8 cap
    Use
    AAV
    Tags
    Expression
    Mutation
    7mer insertion in the AAV2 cap
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64839-plasmid-map-sequence-id-342083

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  5. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-275513

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  7. pT4-CMV-GFP

    Plasmid
    #117046
    Purpose
    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
    Depositor
    Wolfgang Uckert
    Insert
    GFP
    Use
    Transposon
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117046-plasmid-map-sequence-id-258678

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  12. mScarletI-Rab7

    Plasmid
    #112955
    Purpose
    In vivo visualization of Rab7 (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Rab7
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112955-plasmid-map-sequence-id-223575

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  15. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

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  17. pUC57-LbNOX

    Plasmid
    #75285
    Purpose
    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    LbNOX
    Use
    Tags
    C-terminal FLAG tag with linker
    Expression
    Mutation
    Human codon optimized
    Promoter
    Available since
    May 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    75285-plasmid-map-sequence-id-258257

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  18. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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Showing: 1801 - 1820 of 18491 results