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pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…TagsExpressionMutationPromotermyo6bAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet
Plasmid#131001PurposeAAV vector to drive the expression of mScarlet under the control of human Synapsin promoterDepositorHas ServiceAAV1 and AAV8InsertmScarlet
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-PP7
Plasmid#122945PurposePP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particlesDepositorInsertNC-PCP (PP7 coat protein) fusion protein in Gag
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO mScarlet
Plasmid#131002PurposeDouble floxed mScarlet under the control of Ef1a promoterDepositorHas ServiceAAV1 and AAV5InsertmScarlet
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-MS2
Plasmid#122944PurposeMS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particlesDepositorInsertNC-MCP (MS2 coat protein) fusion protein in Gag
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-TBK1
Plasmid#199205PurposeUsed for the expression of TBK1 (SMC Internal No. 1996)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP
Plasmid#223215PurposeLentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertmCherry BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT040
Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 YFP
Plasmid#84910Purposeexpression of YFP in mammalian cellsDepositorInsertYFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-GBF1
Plasmid#206415PurposeMammalian expression of hGBF1DepositorInsertGBF1 (GBF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-Flpo
Plasmid#183535PurposeINTRSECTDepositorInsertFlpo
UseAAVTagsExpressionMutationNonePromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP TMEM41B-GFP
Plasmid#117414PurposeExpresses TMEM41B tagged with GFP in mammalian cellsDepositorInsertTMEM41B (TMEM41B Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Grx1-roCherry
Plasmid#141068PurposeFluorescent biosensor for 2GSH/GSSG ratioDepositorInsertGrx1-roCherry
UseTagsExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threon…PromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCS-TurboID MSCV-IRES-GFP
Plasmid#207957PurposeRetroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminusDepositorInsertTurboID
UseRetroviral; Turboid biotin proximity ligaseTagsTurboIDExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP TMEM41B-3xFLAG
Plasmid#117415PurposeExpresses TMEM41B tagged with 3xFLAG in mammalian cellsDepositorInsertTMEM41B (TMEM41B Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-BLIMP-1-pcDNA3.1-
Plasmid#52518Purposeexpresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertBlimp1 (PRDM1 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only
