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Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
UseTags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-human RNF146 RING-WWE (30-183)
Plasmid#132611Purposeexpresses human RNF146 RING-WWE (30-183) in E. coliDepositorInsertRNF146 RING-WWE (30–183)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLC-mEos2
Plasmid#38010DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsmEos2ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMXs-Ms-c-Myc-W136E
Plasmid#26024DepositorInsertc-Myc (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationW136EPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Bt δCOP
Plasmid#73871PurposeBos taurus Arcn1DepositorInsertRet2 Promoter- COPD
UseTagsExpressionYeastMutationPromoterRet2 PromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
UseTagsExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
prLCA-Citrine
Plasmid#40271DepositorInsertClta (Clta Rat)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pRK-flag-USP19 1-493
Plasmid#78581PurposeExpression flag-USP19 1-493 in mammalian cellsDepositorInsertflag-USP19 1-493 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutated 494 residue to stop codonPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(2,5kb)
Plasmid#14980DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Dlx5
Plasmid#74344PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.DepositorInsertdistal-less homeobox 5 (Dlx5 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Actin-7
Plasmid#55462PurposeLocalization: Actin, Excitation: 462, Emission: 492DepositorInsertActin (ACTB Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IP3KC
Plasmid#32527DepositorInsertIP3KC (ITPKC Human)
UseTags6xHISExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only