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miR3 Mm ATP13A2

Plasmid

#171771

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP_AAVS1

Plasmid

#141211

Purpose

pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA spred1, mitfa:Cas9

Plasmid

#118843

Purpose

Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

spred1 gRNA (spred1 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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LL - hNANOGi

Plasmid

#12196

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

NANOG - RNAi (NANOG Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001144909)

Plasmid

#76890

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.dTomato-miR30n

Plasmid

#90334

Purpose

miR30 based shMir knockdown

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

dTomato
na
SFFV
miR30n

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

Plasmid

#175175

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

sgRNA-lacZ

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b-gRNA-NT

Plasmid

#184568

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFH6_new

Plasmid

#105866

Purpose

more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

Depositor

Andreas Weber

Article

Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Subcloning

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA3-RPB1-N-term

Plasmid

#195112

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA1-RPB1-N-term

Plasmid

#195110

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RACK1

Plasmid

#127123

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RACK1 (RACK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-L3US2-RFP-HDAC3

Plasmid

#83966

Purpose

Lentiviral CRISPR HDAC3 dual gRNA targeting vector

Depositor

Eleanor Chen

Insert

HDAC3 dual gRNAs (HDAC3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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BE3-hA3A-R128A

Plasmid

#132944

Purpose

Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-hA3A(R128A)

Use

CRISPR; Base editor

Tags

Expression

Mammalian

Mutation

BE3-hA3A(R128A)

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001148198)

Plasmid

#77665

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001145736)

Plasmid

#77666

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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