Skip to main content
Addgene
Showing: 161 - 180 of 15679 results

  1. XLone-BSD NFIL3 T2A Puro

    Plasmid
    #140028
    Purpose
    Tunable and temporal expression control of NFIL3 and puro resistant gene
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140028-plasmid-map-sequence-id-275842

    Enlarge

  2. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110404-plasmid-map-sequence-id-307700

    Enlarge

  5. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

    Enlarge

  9. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

    Enlarge

  10. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122182-plasmid-map-sequence-id-237289

    Enlarge

  11. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122183-plasmid-map-sequence-id-241282

    Enlarge

  13. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

    Enlarge

  14. pDual_dsCas9_Puro_hs_TP73

    Plasmid
    #214689
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214689-plasmid-map-sequence-id-428791

    Enlarge

  15. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid
    #195182
    Purpose
    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.
    Depositor
    Stephanie Roessler
    Insert
    NUDT17 (NUDT17 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter and minimal CMV
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195182-plasmid-map-sequence-id-423138

    Enlarge

  16. pTRIPZ-NUDT18-C-FLAG-Puro

    Plasmid
    #195181
    Purpose
    Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.
    Depositor
    Stephanie Roessler
    Insert
    NUDT18 (NUDT18 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    minimal CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195181-plasmid-map-sequence-id-423109

    Enlarge

  18. pSBBi-RP GST-PcCS

    Plasmid
    #139160
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    N-GST caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139160-plasmid-map-sequence-id-309126

    Enlarge

  19. pSBBi-RP GST-CCS1

    Plasmid
    #139164
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST coffee caffeine synthase 1 (GST-CCS1) fusion; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    glutathione transferase + caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139164-plasmid-map-sequence-id-309354

    Enlarge

Showing: 161 - 180 of 15679 results