-
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet
Plasmid#131001PurposeAAV vector to drive the expression of mScarlet under the control of human Synapsin promoterDepositorHas ServiceAAV1 and AAV8InsertmScarlet
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SUN2 mScarlet
Plasmid#187685PurposeIn vivo visualization of SUN2DepositorInsertSUN2 (Sun2 Mouse)
UseTagsmScarletExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_SBE4:mScarlet-I-NLS
Plasmid#154961Purposeencodes transcriptional reporter of SMAD2/3DepositorInsertmScarlet
UsePiggybacTagsNLS-PESTExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Zeo
Plasmid#207733PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Zeo-mScarlet
Plasmid#207700PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
Plasmid#191389PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet
Plasmid#169219PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a mScarlet
Plasmid#178543PurposeThe mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmScarlet
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Puro
Plasmid#207722PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pN1-COX8-mScarletI
Plasmid#129341PurposeMitochondria matrix localizationDepositorInsertmScarlet(i) (Addgene 85044)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mScarlet
Plasmid#207689PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMHE-mScarlet-hCenpC
Plasmid#171490PurposeT7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNADepositorInsertCenpC (CENPC Human)
UsePgehmeTagsmScarletExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Double UP mScarlet to mNeon
Plasmid#125139PurposeNatively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIp10 - mScarlet-I IDT
Plasmid#163120PurposeExpresses in Candida albicansDepositorInsertmScarlet-I IDT
UseCandida albicans expressionTagsExpressionMutationPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 WT
Plasmid#186472PurposeExpression of human MOSPD2 fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialMutationPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mScarlet
Plasmid#207678PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQCXIP mScarlet-MOSPD2 RD/LD
Plasmid#186476PurposeExpression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only