pKUNOC-ErbB1-mScarlet
(Plasmid
#197360)
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PurposeEncoding human ErbB1 tagged with mScarlet.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197360 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKUNOC
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 11000
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Vector typeMammalian Expression
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Selectable markersmScarlet
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameERBB1
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SpeciesH. sapiens (human)
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Insert Size (bp)4300
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
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Tag
/ Fusion Protein
- mScarlet (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKUNOC-ErbB1-mScarlet was a gift from Michiyuki Matsuda (Addgene plasmid # 197360 ; http://n2t.net/addgene:197360 ; RRID:Addgene_197360) -
For your References section:
Knockout of all ErbB-family genes delineates their roles in proliferation, survival, and migration. Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, Matsuda M, Terai K. J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199. 10.1242/jcs.261199 PubMed 37519219