Skip to main content
Addgene
Showing: 161 - 180 of 6897 results

  1. pO3

    Plasmid
    #193748
    Purpose
    Expresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-
    Insert
    E2-Crimson
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PAAH
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193748-plasmid-map-sequence-id-390452

    Enlarge

  2. pO6

    Plasmid
    #193747
    Purpose
    Expresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,
    Depositor
    Sangram Bagh
    Article
    Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-
    Insert
    E2-Crimson
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PIAH
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193747-plasmid-map-sequence-id-390458

    Enlarge

  4. pCm N-term SMT3 NikJC199U

    Plasmid
    #174210
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174210-plasmid-map-sequence-id-342456

    Enlarge

  5. pCm C-term SMT3 NikJC199U

    Plasmid
    #174362
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174362-plasmid-map-sequence-id-342846

    Enlarge

  6. pCm N-term MBP NikJC199U

    Plasmid
    #174364
    Purpose
    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174364-plasmid-map-sequence-id-342851

    Enlarge

  7. pCm C-term MBP NikJC199U

    Plasmid
    #174365
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174365-plasmid-map-sequence-id-342848

    Enlarge

  10. ARK2a

    Plasmid
    #81017
    Purpose
    E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)
    Depositor
    Taek Soon Lee
    Article
    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10
    Inserts
    MevTo-BBa1002-pTrc-Mkco
    PMD
    Use
    Tags
    Expression
    Mutation
    Promoter
    LacUV5 and Trc promoter
    Available since
    Aug. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    81017-plasmid-map-sequence-id-153405

    Enlarge

  11. ARK1a

    Plasmid
    #81016
    Purpose
    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)
    Depositor
    Taek Soon Lee
    Article
    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10
    Inserts
    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    Use
    Tags
    Expression
    Mutation
    Promoter
    LacUV5 and Trc promoter
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81016-plasmid-map-sequence-id-153403

    Enlarge

  12. pJH123

    Plasmid
    #68379
    Purpose
    pJH122 variant Gateway destination
    Depositor
    Trevor Charles
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68379-plasmid-map-sequence-id-127728

    Enlarge

  13. pJH145

    Plasmid
    #68380
    Purpose
    IMCE attB donor copy control fosmid
    Depositor
    Trevor Charles
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    68380-plasmid-map-sequence-id-119857

    Enlarge

  14. pminTolR4-R2

    Plasmid
    #67711
    Purpose
    mini Tol2 backbone with att4-attR2 cassette
    Depositor
    Nathan Lawson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67711-plasmid-map-sequence-id-117439

    Enlarge

  15. pgLAP1

    Plasmid
    #19702
    Depositor
    Peter Jackson
    Type
    Empty backbone
    Use
    Tags
    EGFP, S peptide, and TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    19702-plasmid-map-sequence-id-9330

    Enlarge

  16. pgLAP2

    Plasmid
    #19703
    Depositor
    Peter Jackson
    Type
    Empty backbone
    Use
    Tags
    Flag, S peptide, and TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    19703-plasmid-map-sequence-id-9331

    Enlarge

  17. pTU2S-a (p15A origin)

    Plasmid
    #74088
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74088-plasmid-map-sequence-id-138296

    Enlarge

  18. pS3

    Plasmid
    #50596
    Purpose
    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Inserts
    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    50596-plasmid-map-sequence-id-77162

    Enlarge

  19. pDEINO4

    Plasmid
    #179488
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    aadA1
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179488-plasmid-map-sequence-id-360447

    Enlarge

  20. pDEINO3

    Plasmid
    #179487
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    TetR/A
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179487-plasmid-map-sequence-id-360433

    Enlarge

Showing: 161 - 180 of 6897 results