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pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ptdh3

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS xenopus paraxial protocadherin

Plasmid

#24687

Depositor

Edward De Robertis

Article

Kim et al Development. 1998 Dec . 125(23):4681-90.

Insert

xenopus paraxial protocadherin (pcdh8.2.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_eGFP-pScaD

Plasmid

#228825

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaD

Use

Tags

eGFP

Expression

Mammalian

Mutation

aa 30-700

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-A3 [N144/5R]

Plasmid

#222154

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-A3 (Mouse). Derived from hybridoma N144/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Gamma-protocadherin-A3 (Mus musculus) recombinant mouse monoclonal antibody. (Pcdhga3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tet2puro-gal4DBD-HIFCAD

Plasmid

#207173

Purpose

Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD

Use

Lentiviral; Dox inducible

Tags

2x Myc

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Gamma-protocadherin-A3 scFv [N144/17]

Plasmid

#206739

Purpose

Mammalian Expression of Gamma-protocadherin-A3 scFV. Derived from hybridoma N144/17 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Gamma-protocadherin-A3 (Mus musculus) recombinant scFV (Pcdhga3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-B2 [N148/30]

Plasmid

#190299

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-B2 (Mouse). Derived from hybridoma N148/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Gamma-protocadherin-B2 (Mus musculus) recombinant Mouse monoclonal antibody (Pcdhgb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA1-gltA2-GapAP1

Plasmid

#87150

Purpose

gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

gltA1-gltA2-gapAP1 guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Prps3-CAD-Tcyc1

Plasmid

#127728

Purpose

Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Prps3

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Peft2-CAD-Tklura3

Plasmid

#127729

Purpose

Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Peft2

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Psmtef1-CAD-Tyhi9

Plasmid

#127730

Purpose

Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Psmtef1

Available since

Availability

Academic Institutions and Nonprofits only

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pHJ12: CadC-VHH-Caffeine (No linker)

Plasmid

#108244

Purpose

Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT

Plasmid

#19291

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

E-cadherin

Use

Luciferase

Tags

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125 Ebo…

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pF711-pET28a-Hs-delta25-MCAD-NHis wt

Plasmid

#85112

Purpose

expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta25) MCAD (ACADM Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Gamma-protocadherin-C3 [N174B/27R]

Plasmid

#164156

Purpose

Mammalian Expression Plasmid of anti-Gamma-protocadherin-C3 (Mouse). Derived from hybridoma N174B/27.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Gamma-protocadherin-C3 (Mus musculus) recombinant mouse monoclonal antibody (Pcdhgc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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