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Plasmid#19850DepositorInsertMKL1 L272A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 272 to AlaninePromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
3405 pBM272 Bid p22
Plasmid#8771DepositorInsertBid p22 (Bid Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-mNeon
Plasmid#186605PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsZNF598_1-272_AH
Plasmid#148863PurposeMammalian Expression of HsZNF598_1-272DepositorInsertHsZNF598_1-272 (ZNF598 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW706_pCAG-PV2-lox2272-GFP
Plasmid#87570PurposePV2 for 2-input, 2-output circuit libraryDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD1-L272A
Plasmid#33037DepositorInsertTEAD1 (TEAD1 Human)
UseTagsGal4ExpressionMammalianMutationL272APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-TEAD1-L272A
Plasmid#33050DepositorInsertTEAD1 (TEAD1 Human)
UseTagsMycExpressionMammalianMutationL272APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
1272 pBabe puroL Myr HA Akt3
Plasmid#9019DepositorInsertAkt3 (AKT3 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-lox2272
Plasmid#154833PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanerceptDepositorInsertETN
UseCre/LoxTagsExpressionMammalianMutationPromoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-lox2272
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-mBirA272-NRX3b D137A
Plasmid#43922DepositorInsertBirA272-NRX3b (birA Synthetic, Human)
UseTags2XFLAGExpressionMammalianMutationD137APromoterCAGAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Minos ITR loxp MCS lox2272
Plasmid#218983PurposePlasmid containing ITR sequences compatible with Minos transposase and multiple cloning siteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-CAG-mRFP-PCNA pc2729
Plasmid#166040PurposeExpresses mRFP tagged human PCNA in mammalian cells.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo PFKL-GFP, K272R
Plasmid#138290PurposeMammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP, K272R, fusion proteinDepositorInsertPFKL (PFKL Human)
UseLentiviralTagsGFPExpressionMammalianMutationK272RPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
Plasmid#168787PurposeDonor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.DepositorTypeEmpty backboneUseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP1-loxP-bar-lox2272-71
Plasmid#168785PurposeVector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP2-loxP-bar-lox2272-71
Plasmid#168786PurposeVector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
IS1 Homology arm 1
IS1 Homology arm 2
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS1272 TDP43 RRM-GFP W385L
Plasmid#107809PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationW385L mutation in LLPS reporterPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only