1. K218Tc-3

   Plasmid

   #1300

   Depositor

   Susan Lindquist

   Article

   Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

   Insert

   mutant HSP104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K218T: A315T

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pDEST-ERBB2-v1

   Plasmid

   #154901

   Purpose

   Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST-ERBB2-v2

   Plasmid

   #154895

   Purpose

   Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   Venus fragment 2 (v2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRT.Agro.CLCrVB1.3

   Plasmid

   #37974

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

   Insert

   pJRTCLCrVB1.3
   

   Use

   Agrobacterium binary

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJRT.Agro.CLCrVA.009

   Plasmid

   #37972

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

   Insert

   pJRTCLCrVA.009
   

   Use

   Agrobacterium binary

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJRT.Agro.CLCrVA.010

   Plasmid

   #31810

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

   Insert

   JRTCLCrVA.010
   

   Use

   Agrobacterium binary

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJRT.Agro.CLCrVA.008

   Plasmid

   #31809

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

   Insert

   JRTCLCrVA.008
   

   Use

   Agrobacterium binary

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSOMA-NLS

   Plasmid

   #118937

   Purpose

   Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCambia-PUP-IT

   Plasmid

   #186478

   Purpose

   Expresses POI-PUP-IT fusion in Plants

   Depositor

   Jen Sheen

   Article

   Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

   Insert

   FLAG-Pup(E)
   

   Use

   Tags

   Triple Flag Tag

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS2 (CK2alpha', CK2beta)

    Plasmid

    #27093

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha' (CSNK2A2 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGV7 (CK2alpha' K69M, CK2beta)

    Plasmid

    #27095

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha' K69M (CSNK2A2 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA tag on CK2alpha' and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K69M on CK2alpha'

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIOX

    Plasmid

    #51300

    Purpose

    E.coli expression vector for His8-SUMO tagged recombinant protein

    Depositor

    Andrea Mattevi

    Type

    Empty backbone

    Use

    Tags

    His8 + SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PROM_7

    Plasmid

    #201300

    Purpose

    D7: Promoter of TraesCS3D02G316100 as Golden Gate compatible MoClo PROM part

    Depositor

    Benjamin Schwessinger

    Insert

    D7: Promoter of TraesCS3D02G316100
    

    Use

    Tags

    Expression

    Plant

    Mutation

    removal of BsaI and BpiI sites

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCaSpeR eGFP-dhd

    Plasmid

    #113004

    Purpose

    P element vector for transposon insertion into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CaSpeR dhd 3X FLAG

    Plasmid

    #113002

    Purpose

    P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCaSpeR dhd-eGFP

    Plasmid

    #113003

    Purpose

    P element vector for transposon insertion of dhd-eGFP into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. rag1 short

    Plasmid

    #11300

    Depositor

    Lisa Steiner

    Article

    Willett et al Immunogenetics. 1997 . 45(6):394-404.

    Insert

    rag1 (rag1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

18. PP-PYRK1 (3KHD)

    Plasmid

    #25278

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFF1300w:A10-E511 (PFF1300w Plasmodium falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids A10-E511

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. Sub2 pRS315

    Plasmid

    #111300

    Purpose

    Sub2 in pRS315 vector

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTETDuet_WTDHFR_R127ATS

    Plasmid

    #191300

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT DHFR, R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge