1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

   Plasmid

   #80325

   Purpose

   Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

   Depositor

   Daniel Schoenberg

   Article

   Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

   Insert

   PXDNL-003 (PXDNL Human)
   

   Use

   Tags

   Biotin

   Expression

   Mammalian

   Mutation

   H170A and H256A

   Promoter

   CMV

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

   Plasmid

   #148404

   Purpose

   Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and two non silent N605S, K75…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC

   Plasmid

   #148414

   Purpose

   Mammalian Expression of HsNot1iso1_1318-1605-shRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsNot1iso1_1318-1605-shRNAres (CNOT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1 silent mutation compared to the sequence of iso…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC

   Plasmid

   #148427

   Purpose

   Mammalian Expression of HsAGO2-K663AL697Y

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsAGO2-K663AL697Y (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three silent mutations and the sequence differs i…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC

   Plasmid

   #148428

   Purpose

   Mammalian Expression of HsAGO2-P593GA623FK663AL697Y

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsAGO2-P593GA623FK663AL697Y (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three silent mutations and the sequence differs i…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

   Plasmid

   #148440

   Purpose

   Mammalian Expression of HsAGO2

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsAGO2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C840Tand sequence differs in …

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

   Plasmid

   #148442

   Purpose

   Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1 silent mutation compared to the sequence of iso…

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

   Plasmid

   #148420

   Purpose

   Mammalian Expression of HsRck_296-472-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck_296-472-10xmut (DDX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC

   Plasmid

   #148394

   Purpose

   Insect Expression of DmAGO1-L819Y-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   DmAGO1-L819Y-siRNAres (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC

    Plasmid

    #148395

    Purpose

    Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    variation with 2 silent mutations compared to the…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF5/FRT-DEST EVC2ala4-Flag

    Plasmid

    #41008

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

    Plasmid

    #41011

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pPBpuro-ErbB1(DEL1011)

    Plasmid

    #197366

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant. (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1011-1186

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFOSGE_COF2H02

    Plasmid

    #70877

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    toy (toy Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGS373_tdTomato-1xGCN4-T2A-BSD Lenti

    Plasmid

    #211869

    Purpose

    1xGCN4pep transgene expression

    Depositor

    Iain Cheeseman

    Insert

    1xGCN4pep
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNM220_AAVS1 integration helper

    Plasmid

    #211864

    Purpose

    Cas9 cutting at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGS96_EGFP(A206K)-I3-01 AAVS donor

    Plasmid

    #211865

    Purpose

    I3-01 transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    I3-01
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211860

    Purpose

    CDS for E3-DART ligase sequence in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD2-HA-LRR-NELC492A-C (B3-B4)

    Plasmid

    #211862

    Purpose

    Mutant E3-DART ligase in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mutation

    C492A

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge