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Plasmid#193941PurposeMammalian expression of tdTomato-tagged PEX3DepositorInsertPEX3 (PEX3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY37
Plasmid#191041Purposesrsx-3 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertsrsx-3 (srsx-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha2
Plasmid#165859PurposeAlpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega2
Plasmid#165861PurposeOmega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA-ΔN335-Kin1A
Plasmid#31606DepositorInsertΔN335 kinesin-1A (Kif5a Mouse)
UseTagsHA-tagExpressionMammalianMutationdeleted amino acids 1-335PromoterCMVAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1spm
Plasmid#165866PurposeModified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega2
Plasmid#165865PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYC-mTET1-N
Plasmid#81056Purposebacterial expression of N-terminus of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationN-terminus, amino acids 301-1366PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D2
Plasmid#61491Purposeluciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A-SNAPf-HisTag-FLAG
Plasmid#200789PurposeMammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
KIF5A-SNAPf-HisTag-FLAG
Plasmid#200791PurposeMammalian expression of KIF5A-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsSNAPf, HisTag, FLAGExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1R+10
Plasmid#190844PurposeFor mammalian expression of 3JB1R+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1R_AP3+10bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-3JB1F+10
Plasmid#190837PurposeFor mammalian expression of 3JB1F+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+10bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
H2A-mTurquoise-RhoJQ79L-deltaCaax
Plasmid#176110PurposeConstitutively active, nucelar localized RhoJDepositorInsertRhoJ (RHOJ Human)
UseTagsExpressionMammalianMutationQ79LPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pColE1-AlphaOmega
Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha1
Plasmid#165858PurposeAlpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega1
Plasmid#165860PurposeOmega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only