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Showing: 401 - 450 of 1133 results

  4. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  5. pDONR221-SLC4A1_STOP

    Plasmid
    #161346
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC4A1 (SLC4A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161346-plasmid-map-sequence-id-338285

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  7. pD649-HAsp-SLAMF9-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156648
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SLAMF9 (SLAMF9 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156648-plasmid-map-sequence-id-444411

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  8. pD649-HAsp-CD80-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156625
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD80 (CD80 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156625-plasmid-map-sequence-id-424368

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  10. pTT3-ecdCD80-ST3-His

    Plasmid
    #210665
    Purpose
    Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210665-plasmid-map-sequence-id-443131

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  12. pD649-HAsp-FCER2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156716
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCER2 (FCER2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156716-plasmid-map-sequence-id-302541

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  15. pDONR221-RHAG_STOP

    Plasmid
    #161037
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    RHAG (RHAG Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161037-plasmid-map-sequence-id-312008

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  16. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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  17. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

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  18. hTLR4

    Plasmid
    #13086
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13086-plasmid-map-sequence-id-142442

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  19. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13642-plasmid-map-sequence-id-392328

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  20. pcDNA3-TLR10-YFP

    Plasmid
    #13643
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 10 (TLR10 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13643-plasmid-map-sequence-id-7721

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  21. pcDNA3-TLR6-YFP

    Plasmid
    #13020
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 6 (TLR6 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13020-plasmid-map-sequence-id-7420

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  22. pcDNA3-TLR6-CFP

    Plasmid
    #13021
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 6 (TLR6 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13021-plasmid-map-sequence-id-7421

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  23. pcDNA3-TLR3-CFP

    Plasmid
    #13641
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 3 (TLR3 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13641-plasmid-map-sequence-id-7719

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  24. pcDNA3-TLR8-YFP

    Plasmid
    #13024
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 8 (TLR8 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13024-plasmid-map-sequence-id-7422

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  25. pGL3_MSR1

    Plasmid
    #232391
    Purpose
    chr8:16099746-16100677 cloned into pGL3
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232391-plasmid-map-sequence-id-466686

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  26. p75-RFP

    Plasmid
    #24092
    Depositor
    Moses Chao
    Insert
    p75NTR (NGFR Human)
    Use
    Tags
    DSRed
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    24092-plasmid-map-sequence-id-210141

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  27. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

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  28. hTLR3 flag

    Plasmid
    #13084
    Depositor
    Ruslan Medzhitov
    Insert
    TLR3 (TLR3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-17 have been replaced by mammalian …
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13084-plasmid-map-sequence-id-229543

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  29. hTLR1 flag

    Plasmid
    #13081
    Depositor
    Ruslan Medzhitov
    Insert
    TLR1 (TLR1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-18 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  31. TLR10A

    Plasmid
    #42364
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TLR10A (TLR10 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pGL3_MSR1_bothneg0scramble

    Plasmid
    #232383
    Purpose
    MSR1 enhancer, both buffering Coordinators scrambled
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, both buffering Coordinators scramb…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232383-plasmid-map-sequence-id-462848

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  33. pMINTBeta1

    Plasmid
    #14127
    Depositor
    Richard Hynes
    Insert
    Mouse Beta 1 Integrin (Itgb1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only

  34. R777-E007 Hs.ALK

    Plasmid
    #70291
    Purpose
    Gateway ORF clone of human ALK [NM_004304.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ALK (ALK Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70291-plasmid-map-sequence-id-125740

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  35. R777-E105 Hs.INSR

    Plasmid
    #70389
    Purpose
    Gateway ORF clone of human INSR [NM_001079817.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    INSR (INSR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70389-plasmid-map-sequence-id-125838

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  36. pGL3_MSR1_rightneg0topos1

    Plasmid
    #232389
    Purpose
    MSR1 enhancer, right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, right buffering Coordinator to sen…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232389-plasmid-map-sequence-id-462852

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  37. PLXNC1

    Plasmid
    #25312
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXNC1:T1198-K1305 (PLXNC1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids T1198-K1305
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. R777-E106 Hs.INSR-nostop

    Plasmid
    #70390
    Purpose
    Gateway ORF clone of human INSR [NM_001079817.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    INSR (INSR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70390-plasmid-map-sequence-id-125839

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  39. R777-E008 Hs.ALK-nostop

    Plasmid
    #70292
    Purpose
    Gateway ORF clone of human ALK [NM_004304.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ALK (ALK Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70292-plasmid-map-sequence-id-125741

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  40. pGL3_MSR1_leftneg0topos3

    Plasmid
    #232385
    Purpose
    MSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, left buffering Coordinator convert…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232385-plasmid-map-sequence-id-462871

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Showing: 401 - 450 of 1133 results