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PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMIA II
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Backbone manufacturerDario Vignali, Addgene plasmid 52107
- Total vector size (bp) 8632
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCD3 delta
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Alt nameCD3 antigen, delta polypeptide
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SpeciesM. musculus (mouse)
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Entrez GeneCd3d (a.k.a. T3d)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCDC gamma
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Alt nameCd3e CD3 antigen, gamma polypeptide
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SpeciesM. musculus (mouse)
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Entrez GeneCd3g (a.k.a. Ctg-3, Ctg3, T3g)
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Tag
/ Fusion Protein
- F2A (N terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameCD3 epsilon
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Alt nameCd3e CD3 antigen, epsilon polypeptide
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SpeciesM. musculus (mouse)
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Entrez GeneCd3e (a.k.a. CD3, CD3epsilon, T3e)
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Tag
/ Fusion Protein
- T2A (N terminal on insert)
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameCD3 zeta
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Alt nameCd247
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SpeciesM. musculus (mouse)
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Entrez GeneCd247 (a.k.a. 4930549J05Rik, A430104F18Rik, Cd3, Cd3-eta, Cd3-zeta, Cd3h, Cd3z, Cd3zeta, T3z, Tcrk, Tcrz)
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Tags
/ Fusion Proteins
- P2A (N terminal on insert)
- IRES-EGFP (C terminal on backbone)
Cloning Information for Gene/Insert 4
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II was a gift from Dario Vignali (Addgene plasmid # 52093 ; http://n2t.net/addgene:52093 ; RRID:Addgene_52093) -
For your References section:
Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Holst J, Wang H, Eder KD, Workman CJ, Boyd KL, Baquet Z, Singh H, Forbes K, Chruscinski A, Smeyne R, van Oers NS, Utz PJ, Vignali DA. Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 May 11. 10.1038/ni.1611 PubMed 18469818