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Plasmid#64596PurposeExpression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentationDepositorInsertOvalbumin
UseTagsInvariant chainExpressionMammalianMutationOnly amino acids 242-353 (class I and class II epā¦PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK283 (Stag-3FLAG-Neo)
Plasmid#72799PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Neo
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-LASP2-C-18
Plasmid#54143PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertLASP2 (NEBL Chicken)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-PK
Plasmid#24163DepositorInsertpyruvate kinase
UseTagsGFPExpressionMammalianMutationn/aPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK30
Plasmid#59121PurposeFAK fret sensor with 30 nm helixDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8m-WPRE
Plasmid#176757PurposeAAV-mediated expression of GCaMP8m under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-YTB
Plasmid#26721DepositorInsertUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68717PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readiā¦PromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Vinculin
Plasmid#80024PurposeMammalian expression of mCherry-vinculinDepositorInsertvinculin (VCL Chicken)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
Plasmid#104493PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV Retrograde and AAV1InsertjGCaMP7b
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO2-TVA-mCherry
Plasmid#149297PurposeCre-dependent expression of TVA:mCherry fusion protein for rabies tracing.DepositorInsertTVA:mCherry
UseAAVTagsExpressionMutationStart codon removed.PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcU6_1 sgRNA
Plasmid#92395PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.DepositorInsertchick U6.1 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.1Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68720PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readiā¦PromoterhSyn1-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE
Plasmid#104497PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorInsertjGCaMP7b
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8f
Plasmid#169249PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE
Plasmid#105322PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV1InsertjGCaMP7c variant 1513
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK277 (mClover-NeoR)
Plasmid#72793PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Neo
UseCRISPRTagsmCloverExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8f
Plasmid#167573PurposePlease see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.DepositorInsertRibo-GCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only