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Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:KIT L576P
Plasmid#118848PurposeExpresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertKIT L576P (KIT Zebrafish, Human)
UseZebrafish expression, tol4TagsExpressionMutationKIT L576PPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-TagBFP
Plasmid#117771PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
Plasmid#105643PurposeCo-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-Gal4-Vp16-Crys-CFP
Plasmid#97144PurposeFor Gal4/UAS expression systemDepositorInsertGal4-Vp16
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-Isce1-eno2:egfp
Plasmid#61394PurposeExpresses GFP in neurons of transgenic zebrafishDepositorInserteno2 regulatory element
UseZebrafish expressionTagsExpressionMutationPromotereno2Available sinceDec. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-krt5
Plasmid#82585Purpose5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expressionDepositorInsertzebrafish krt5 promoter (krt5 Zebrafish)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYC-97
Plasmid#31565DepositorInsertpCS4+-NLS-EGFP-P2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-zTRAP
Plasmid#42236DepositorInsertEGFP-Rpl10a (rpl10a Zebrafish)
UseTagsEGFPExpressionMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab32a
Plasmid#109621PurposeMuscle specific zebrafish expression of mKate2 fused to rab32aDepositorInsertrab32a (rab32a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ZfCry4
Plasmid#136242PurposeProtein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tagDepositorInsertZfCry4
UseTagsFlagExpressionMammalianMutationD196H- Please see depositor comments belowPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only