-
Plasmid#172374PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + iCre
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-mDLX-TVA-2A-N2cG
Plasmid#172365PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermDLXAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p45)
Plasmid#135942PurposeExpresses AUF1p45 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-NCwt-SYFP2
Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2
Plasmid#113890PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2
Plasmid#113889PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-FlicR1
Plasmid#74143PurposeExpress FlicR1 preferentially in neuronsDepositorInsertFlicR1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-LZ-CD95L
Plasmid#104349PurposeExpresses human CD95L leucine zipper in mammalian cellsDepositorInsertCD95L leucine zipper (FASLG Human)
UseTagsoctahistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hFOXH1
Plasmid#59183PurposeForced expression of human FOXH1DepositorInsertFOXH1 (FOXH1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3a
Plasmid#111055PurposeExpresses dLight1.3a in mammalian cellsDepositorInsertdLight1.3a
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-CaMKII-TVA-P2A-N2cG
Plasmid#172362PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA-ER
Plasmid#113897PurposeMammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-APEX2m
Plasmid#176843PurposeExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-APEX2m
UseIntegratingTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-jRGECO1a
Plasmid#172386PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + jRGECO1a
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRTagsExpressionYeastMutationPromoterSNR52pAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only