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pCSC-NLS-EGFP

Plasmid

#221811

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NLS-EGFP

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP-ccdB

Plasmid

#14314

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBa-MARK2-eGFP

Plasmid

#66706

Purpose

mammalian expression vector for MARK2 with a C-terminal eGFP

Depositor

Gary Banker, Marvin Bentley

Insert

MARK2 (MARK2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-eGFP

Plasmid

#116926

Purpose

Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

Plasmid

#169332

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-ccdB-EGFP

Plasmid

#14202

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-2E2-HA-mEGFP

Plasmid

#129595

Purpose

The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-NDP52

Plasmid

#175749

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2

Depositor

Wade Harper

Article

Heo et al Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10

Insert

NDP52/CALCOCO2 (CALCOCO2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 EGFP-TEADi

Plasmid

#140145

Purpose

Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH

Plasmid

#225475

Purpose

Express mEGFP-fusion protein of GAPDH

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

U6

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPABPC1_I

Plasmid

#146542

Purpose

Mammalian Expression of HsPABPC1

Depositor

Elisa Izaurralde

Insert

HsPABPC1 (PABPC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFX-mito-EGFP

Plasmid

#159096

Purpose

Express EGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitochondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RUNX1-WT

Plasmid

#194574

Purpose

Mammalian Expression of mEGFP-RUNX1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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