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pCMV6-MPP8-W80A-10xHis

Plasmid

#194179

Purpose

Expresses MPP8-W80A_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-W80A inside chromo domain

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Use

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-HsPatL1_70-383_K

Plasmid

#146725

Purpose

Mammalian Expression of HsPatL1_70-383

Depositor

Elisa Izaurralde

Insert

HsPatL1_70-383 (PATL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-HsRCK-mut1_K

Plasmid

#146727

Purpose

Mammalian Expression of HsRck-mut1

Depositor

Elisa Izaurralde

Insert

HsRck-mut1 (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagC-PrP-SBP_L

Plasmid

#146810

Purpose

Mammalian Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP_L

Plasmid

#146812

Purpose

Mammalian Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Armc12/FLAG/BioID2/HA

Plasmid

#180498

Purpose

Expression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-Zdk1-MCS

Plasmid

#178873

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

miRFP670-Zdk1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid1

Plasmid

#173469

Purpose

N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid2

Plasmid

#173470

Purpose

N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid3

Plasmid

#173471

Purpose

N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-124

Plasmid

#171558

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-124

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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