Addgene

We narrowed to 23,388 results for: gfp

Showing: 1741 - 1760 of 23388 results

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Ub-G76V-GFP

Plasmid

#11941

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-G76V-GFP

Use

Tags

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Use

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP:ALFA

Plasmid

#219642

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8-2A-GFP

Plasmid

#82712

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

LTR

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV GFP1-9

Plasmid

#182244

Purpose

Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP 1-9 OPT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-SF2

Plasmid

#17990

Depositor

Tom Misteli

Article

Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

Insert

SF2 (SRSF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains SF2 (aa1-210)

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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mito-meGFP

Plasmid

#172481

Purpose

meGFP targeted to the mitochondrial matrix with a human COX8 presequence

Depositor

Thomas Schwarz

Insert

Mito

Use

Tags

meGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATF6

Plasmid

#32955

Purpose

For visualization of GFP-ATF6 in mammalian cells.

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

M67L and M313I

Promoter

CMV

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFPC1 MOSPD1

Plasmid

#226404

Purpose

Expression of human MOSPD1 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Insert

MOSPD1 (MOSPD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_GFP

Plasmid

#210638

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP

Plasmid

#85482

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Use

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Feng Zhang, Phil Sharp

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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