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Plasmid#113884PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterpHR-CMV-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2
Plasmid#113885PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-Clover-BlastR
Plasmid#138569PurposeUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJDepositorInsertClover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-A2BR
Plasmid#37202DepositorInsertA2BR (ADORA2B Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorInsertLGC-53 (lgc-53 Synthetic, Nematode)
UseTagsExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.2
Plasmid#111054PurposeExpresses dLight1.2 in mammalian cellsDepositorInsertdLight1.2
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.4
Plasmid#111057PurposeExpresses dLight1.4 in mammalian cellsDepositorInsertdLight1.4
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-RILPL1
Plasmid#201470PurposeFor overexpression in cultured cells to visualize RILPL1 protein interactions.DepositorInsertRILPL1 (RILPL1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-mVenus-Twin-Strep
Plasmid#113891PurposeMammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusionDepositorTypeEmpty backboneUseLentiviralTags3C-mVenus-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10316:GABR1
Plasmid#157850PurposeHA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expressionDepositorInsertGABR1 (GABBR1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mRuby2
Plasmid#185008PurposeFor knocking in FKBP F36V P2A mRuby2DepositorInsertFKBP F36V P2A mRuby2
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
Plasmid#83491Purposecre-dependent DN-AMPK under EF1a promoterDepositorInsertDN-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationK45RPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA
Plasmid#113896PurposeMammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-nLight3.1
Plasmid#111061PurposeExpresses nLight3.1 in mammalian cellsDepositorInsertnLight3.1
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only