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Plasmid#216350PurposexhNup35-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC227
Plasmid#216351PurposexhNup35-Nb1t (+3Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04477
Plasmid#216352PurposexhNup35-Nb1t Nanobody productionDepositorInsertxhNup35-Nb1t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEYFP_C1_FANCM
Plasmid#221384PurposeExpresses FANCM in mammalian cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL850
Plasmid#214250PurposeExpression TadCBEa in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL851
Plasmid#214251PurposeExpression TadCBEd in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL852
Plasmid#214252PurposeExpression TadCBEd_V106W in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL853
Plasmid#214253PurposeExpression TadDE in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL854
Plasmid#214254PurposeExpression eTd-CBE in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL855
Plasmid#214255PurposeExpression Td-CBEmax in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL856
Plasmid#214256PurposeExpression TadDE-NG in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 BRCT domain deletion
Plasmid#207110PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.DepositorInsertHaloTag-MDC1 BRCT domain deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 2
Plasmid#207610PurposesgRNA 2 to knockout TR by replacement with a PuroR cassetteDepositorInsertTCAGGCCGCAGGAAGAGGAA
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
UseTagsNoneExpressionMammalianMutationPromoterSV40Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-nCas9n
Plasmid#217487PurposeExpression of nuclear nCas9nDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 1
Plasmid#207609PurposesgRNA 1 to knockout TR by replacement with a PuroR cassetteDepositorInsertACCCTAACTGAGAAGGGCGT
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC
Plasmid#213398Purposetranscriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to CitrineDepositorInsertFingR for endogenous PSD95, transcriptinally regulated
UseAAVTagsCitrineExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIBY55
Plasmid#213707PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:GFP-LT
Plasmid#68996Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsGFP-LTExpressionMutationPromotermhc2dabAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D-HA
Plasmid#209234PurposeMammalian expression of ATP10DDepositorInsertATP10D (ATP10D Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mCasp1
Plasmid#208781PurposeInducibly expresses mouse caspase1 fused to light activatable Cry2DepositorInsertmCherry mCaspase1 Cry2 (Casp1 Mouse)
UseLentiviralTagsmCherry, Cry2ExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT348
Plasmid#204515PurposeBacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hEFA6C
Plasmid#206414PurposeMammalian expression of hEFA6CDepositorInsertEFA6C (PSD2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP1-2
Plasmid#203837PurposeLuciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked outDepositorInsertdAP1-2
UseTagsExpressionMammalianMutationKO of both AP1 sitesPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH50 - [PVQp | gfp | tbb-2 UTR]
Plasmid#200326Purposegfp BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT255-ameRS
Plasmid#202309PurposeameRS repressor plasmid for LPattB2 integrationDepositorInsertameRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT252-amtR
Plasmid#202307PurposeamtR repressor plasmid for LPattB2 integrationDepositorInsertamtR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT251-bm3R1
Plasmid#202306Purposebm3R1 repressor plasmid for LPattB2 integrationDepositorInsertbm3R1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
plYJP067-PcymRC_v2
Plasmid#202302PurposePCymRC_v2-YFP plasmid for LPattB2 integrationDepositorInsertPCymRC_v2-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT243-Pcin
Plasmid#202301PurposePCin-YFP plasmid for LPattB2 integrationDepositorInsertPcin-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
plYJP067-Pttg
Plasmid#202300PurposePTtg-YFP plasmid for LPattB2 integrationDepositorInsertPTtg-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT238-Pbad
Plasmid#202298PurposePBad-YFP plasmid for LPattB2 integrationDepositorInsertPbad-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT237-Ptac
Plasmid#202297PurposePTac-YFP plasmid for LPattB2 integrationDepositorInsertPTac-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT475
Plasmid#202292PurposeRPU plasmid w/ high RBS (p15a backbone)DepositorInsertJ23101-RiboJ00-B0034-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT318
Plasmid#204508Purposerpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematodeDepositorInsertrpl-21p::2xHA::C04F12.1 (C. elegans)
UseTags2xHAExpressionWormMutationPromoterrpl-21 (C. elegans)Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT246
Plasmid#204507Purposecsr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematodeDepositorInsert2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)
UseTags2xFLAGExpressionWormMutationPromotercsr-1 (C. elegans)Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT334
Plasmid#204516PurposeBacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of coh-3 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT186
Plasmid#204513PurposeBacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertPAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT521
Plasmid#204520PurposeBacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT520
Plasmid#204519PurposeBacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT342
Plasmid#204514PurposeBacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertN-terminal fragment of syp-1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT320
Plasmid#204517PurposeBacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertInternal fragment of him-1 (C. elegans) (him-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP1
Plasmid#203835PurposeLuciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 siteDepositorInsertdAP1
UseTagsExpressionMammalianMutationKO of AP1 upstreamPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E1_dAP2
Plasmid#203836PurposeLuciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 siteDepositorInsertdAP1
UseTagsExpressionMammalianMutationKO of AP1 downstreamPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3
Plasmid#201532PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3
UseSynthetic BiologyTagsExpressionMutationPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-NAD-NLS3x
Plasmid#193822PurposeCMV overexpression of ChemoG-NAD in the nucleus of mammalian cell linesDepositorInsertChemoG-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-ChemoL-NAD-NLS3x
Plasmid#193828PurposeCMV overexpression of ChemoL-NAD in the nucleus of mammalian cell linesDepositorInsertChemoL-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD
Plasmid#193829PurposeCMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell linesDepositorInsert[4xCox8]-ChemoL-NAD
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB
Plasmid#193808PurposeCMV overexpression of ChemoB in mammalian cell linesDepositorInsertChemoB
UseTags10xHisExpressionMammalianMutationEBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only