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Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPRIME-CMV-GFP-FF3
Plasmid#11663Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
SIRT6 Flag
Plasmid#13817PurposeMammalian expression of human SIRT6 with flag tagDepositorInsertSIRT6 (SIRT6 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6
Plasmid#32955PurposeFor visualization of GFP-ATF6 in mammalian cells.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationM67L and M313IPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-TFEB-WT-MYC (#647)
Plasmid#99955Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertp53-binding site (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CAPN2
Plasmid#60942PurposeExpresses calpain2 in mammalian cellsDepositorInsertCalpain 2 (CAPN2 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFkB-TA-LUC-UBC-GFP-W
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA-ctrl
Plasmid#85741PurposeshRNA ctrlDepositorInsertshRNA ctrl
UseAAVTagsEYFPExpressionMutationPromoterAvailable sinceApril 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP1-10
Plasmid#68715PurposeSplit GFP assayDepositorInsertGFP1-10
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5xATF6-GL3
Plasmid#11976PurposeReporter gene with five ATF6 sites that is highly sensitive to ER stressDepositorInsert5xATF6 binding site
UseTagsLuc and c-fos promoterExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-DFCP1
Plasmid#86746PurposeN-terminal mCherry-tagged protein expression in mammalian cellsDepositorInserthuman DFCP1 (ZFYVE1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK2
Plasmid#23915DepositorInsertJAK2 (JAK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only