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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TinyERRE

Plasmid

#208320

Purpose

Bacterial expression of the His-tagged peptide TinyERRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TinyERRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh

Plasmid

#208276

Purpose

Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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aCE89

Plasmid

#204494

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

φC31 integrase
WBeta recombinase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

PhIF and TREtight

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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aCE90

Plasmid

#204495

Purpose

TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

phiC31 recombinase
WBeta recombinase

Use

Synthetic Biology

Tags

PEST

Expression

Bacterial and Mammalian

Mutation

Promoter

PhIF and TREtight

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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aCE106

Plasmid

#204496

Purpose

Small molecule helper circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

rtTA3
PhIF-ABI
VPR-Pyl1

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3-mRuby3

Plasmid

#203490

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1_CSL3

Plasmid

#200173

Purpose

Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Article

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

CSL3

Use

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1_SUL-I

Plasmid

#200172

Purpose

Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Article

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

SUL-I

Use

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-mCBP80

Plasmid

#192313

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-mCBP80

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-IBB

Plasmid

#192314

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-IBB

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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