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Nephrin

Plasmid

#186134

Purpose

For expression of recombinant nephrin in bacteria cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NPHS1 (NPHS1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Amino acid residues 1174-1223, with mutations: Y1…

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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3349 pSFFV-neo Bcl-2

Plasmid

#8769

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Jay Keasling, Paul Adams

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23107

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Nck

Plasmid

#186133

Purpose

For expression of recombinant Nck in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NCK1 (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acid residues 1-377, with mutations: C139S,…

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV MEKK3 PB1

Plasmid

#12185

Depositor

Gary Johnson

Article

Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

Insert

MEKK3 (Map3k3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

PB1 domain: aa 36-127

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 deltaCC

Plasmid

#32827

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

E5D, G858D, deletion of amino acids 410-751

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pLNHA-C1-HsUPF2-siRNAres_I

Plasmid

#146567

Purpose

Mammalian Expression of HsUPF2-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF2-siRNAres (UPF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#2

Plasmid

#183120

Purpose

pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EPAS1-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TTRmiRc2

Plasmid

#25754

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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P303-FBP_2

Plasmid

#139693

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE2 - wt)

Plasmid

#16599

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pDGE8

Plasmid

#79463

Purpose

sgRNA shuttle vector; module 2E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe hygro myc p110-CAAX

Plasmid

#12591

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

p110-CAAX (PIK3CA Bovine)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S454A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only