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Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
TAL3438
Plasmid#47157DepositorInsertZebrafishCommunity-fastkd3-left (im:7147223 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlum
Plasmid#119829PurposeProduction of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlum
UseTagsGSTExpressionBacterialMutationR24KPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
FAB(mito-LD)
Plasmid#214425PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito
UseTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.1
Plasmid#111053PurposeExpresses dLight1.1 in mammalian cellsDepositorInsertdLight1.1
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorInsertHDAC8 (HDAC8 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP
Plasmid#113888PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3
Plasmid#189752PurposeProduces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3b
Plasmid#111056PurposeExpresses dLight1.3b in mammalian cellsDepositorInsertdLight1.3b
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-LD)
Plasmid#214420PurposeDetection of ER-lipid droplet contact siteDepositorInsertRspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G
Plasmid#216111PurposeExpresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.DepositorInsertoROS-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6
Plasmid#113887PurposeMammalian (inducible) protein expression, in vivo biotinylationDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-Ef1a-TVA-2A-N2cG
Plasmid#172360PurposeTargeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G
Plasmid#216115PurposeEncodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FRT-EF1a-TVA-2A-N2cG
Plasmid#172361PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only