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pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pSerOTS-C1* (V70)
Plasmid#188537PurposePhosphoserine incorporation into recombinant proteinsDepositorInsertspSerRS
Ef-pSer
tRNApSer
UseTagsExpressionBacterialMutationmutations resulting from laboratory evolutionPromoterglnS*Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
UseTagsExpressionBacterialMutationL270G, Y271L, N311G, C313APromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY
Plasmid#64915PurposeEncodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertsPolyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6/H1Available sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTECH-MmAcK3RS(IPYE)
Plasmid#104071PurposeExpression plasmid encoding MmAcK3RS(IPYE) and pylTDepositorInsertPlpp MmAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LRS-L-11
Plasmid#197720PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LRSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-Cre-T2A-miRFP670
Plasmid#193581PurposeFluorescent reporter for Gal4-induced Cre recombinationDepositorInsertmiRFP670
UseCre/LoxTagsT2AExpressionMutationPromoter5X UASAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only
