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Plasmid#179556Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only
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GCN5 core-dCas9-CBP core
Plasmid#179555Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p300 core-dCas9-CBP core
Plasmid#179559Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
Plasmid#192705PurposePlasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728DepositorUseTags-GFP-TEV-StrepII and 10x-His-TEVExpressionInsectMutationF32A-I35A-I36APromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1-C-LAMP2 TM
Plasmid#183645PurposeLentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.DepositorUseLentiviralTagsHAExpressionMutationATP6AP1 transmembrane domain replaced by the tran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CBP core-dCas9-p300 core
Plasmid#179558Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Fusion + CBP TAZ1(340-439)
Plasmid#173762PurposeBacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-FusionL21A + CBP TAZ1(340-439)
Plasmid#173763PurposeBacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIsGB1-FusionL63A + CBP TAZ1(340-439)
Plasmid#173764PurposeBacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Beta
Plasmid#177962PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)DepositorInsertSARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
UseTagsExpressionMammalianMutationK417N, E484K, N501Y, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-CLEC16A-EMP2
Plasmid#69468PurposeExpresses CLEC16A-EMP2 fusion geneDepositorUseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Epsilon
Plasmid#177964PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)DepositorInsertSARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
UseTagsExpressionMammalianMutationS13I, W152C, L452R, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-K417N
Plasmid#177969PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)DepositorInsertSARS-CoV-2 Spike protein (D614G, K417N) (S )
UseTagsExpressionMammalianMutationD614G, K417NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N439K
Plasmid#177970PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)DepositorInsertSARS-CoV-2 Spike protein (D614G, N439K) (S )
UseTagsExpressionMammalianMutationD614G, N439KPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-L452R
Plasmid#177971PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)DepositorInsertSARS-CoV-2 Spike protein (D614G, L452R) (S )
UseTagsExpressionMammalianMutationD614G, L452RPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-S477N
Plasmid#177972PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)DepositorInsertSARS-CoV-2 Spike protein (D614G, S477N) (S )
UseTagsExpressionMammalianMutationD614G, S477NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N501Y
Plasmid#177975PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)DepositorInsertSARS-CoV-2 Spike protein (D614G, N501Y) (S )
UseTagsExpressionMammalianMutationD614G, N501YPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-S13I
Plasmid#177965PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)DepositorInsertSARS-CoV-2 Spike protein (D614G, S13I) (S )
UseTagsExpressionMammalianMutationD614G, S13IPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-T20N
Plasmid#177966PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)DepositorInsertSARS-CoV-2 Spike protein (D614G, T20N) (S )
UseTagsExpressionMammalianMutationD614G, T20NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-P26S
Plasmid#177967PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)DepositorInsertSARS-CoV-2 Spike protein (D614G, P26S) (S )
UseTagsExpressionMammalianMutationD614G, P26SPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only