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Plasmid#160285PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC144APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-MERS-CoV-3CL-C148A
Plasmid#160283PurposeAllows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC148APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL-C143A
Plasmid#160289PurposeAllows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC143APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterMUT
Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-RFP-GGA3 NGAT
Plasmid#162031PurposeExpresses NGAT sequence of GGA3DepositorInsertGGA3 NGAT (GGA3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Fascin-Promoter 402 mutation
Plasmid#89825PurposeFascin promoter for luciferase assayDepositorInsertFascin1 (FSCN1 Human)
UseLuciferaseTagsExpressionMutation-370 Smad binding element mutated from CAGAC to T…PromoterSV40Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC10
Plasmid#219716PurposeDV2 stable E dimer with fusion loop mutationDepositorInsertDV2 sE SC10
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, T280PPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(3G)-mCherry
Plasmid#206945PurposeMammalian expression of heterobifunctional SasG linkers (3G)DepositorInsertsfGFP-SaSasG (3G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TGA-rt9us-antiGFP
Plasmid#206954PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(7G)-mCherry
Plasmid#206949PurposeMammalian expression of heterobifunctional SasG linkers (7G)DepositorInsertsfGFP-SaSasG (7G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(6G)-mCherry
Plasmid#206948PurposeMammalian expression of heterobifunctional SasG linkers (6G)DepositorInsertsfGFP-SaSasG (6G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(5G)-mCherry
Plasmid#206947PurposeMammalian expression of heterobifunctional SasG linkers (5G)DepositorInsertsfGFP-SaSasG (5G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(4G)-mCherry
Plasmid#206946PurposeMammalian expression of heterobifunctional SasG linkers (4G)DepositorInsertsfGFP-SaSasG (4G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-DCK-Hygro
Plasmid#202409PurposegRNA expression vector for DCKDepositorInsertguide RNA (DCK Human)
UseCRISPR and LentiviralTagsDckExpressionMutationPromoterU6Available sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG
Plasmid#206956PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulinsDepositorInsertmScarlet-I, MmMT3, QtEnc
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TAA-rt20s-antiGFP
Plasmid#206955PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1M-Qt-FLAG-TGA-rt20s-antiGFP
Plasmid#206953PurposeMammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)DepositorInsertMmMT3, QtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(2G)-mCherry
Plasmid#206944PurposeMammalian expression of heterobifunctional SasG linkers (2G)DepositorInsertsfGFP-SaSasG (2G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1
Plasmid#196890PurposeNeuron-specific expression of human (h)Centrin fused to mNeonGreenDepositorInsertmNeonGreen-hCentrin1 (CETN1 Human)
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD
Plasmid#196878PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only