Addgene

We narrowed to 4,918 results for: pcDNA3.1+

Showing: 1701 - 1720 of 4918 results

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Use

Tags

TwinStrep-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP8

Plasmid

#166795

Purpose

Mammalian cell expression vector for FLAG-tagged DPP8

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP8 (DPP8 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

D614G

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-tvD-Cit-tevs-DHFR

Plasmid

#116054

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

Plasmid

#160740

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(C141S)-EGFP

Plasmid

#89635

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) R522D V5 His

Plasmid

#119289

Purpose

CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

R522D

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

Plasmid

#69905

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Use

Tags

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

Plasmid

#164011

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

Plasmid

#69900

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (stw, CDR1-AS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X (G124L, I127A)

Use

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mas-GRK2RH-Nluc L118A

Plasmid

#158606

Purpose

Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614-trunc

Plasmid

#158076

Purpose

Expresses SARS-CoV-2 spike protein with a truncation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

Plasmid

#16752

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-trunc before DEP (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

truncated before DEP

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

Plasmid

#171429

Purpose

Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C261

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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