1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pCR8_TtNAM-B1(1-215)

   Plasmid

   #124577

   Purpose

   NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TtNAM-B1 (1-215)
   

   Use

   Topo gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOCC176

   Plasmid

   #118891

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-C1-TGAT(C242S,C253S)

   Plasmid

   #84336

   Purpose

   Expresses TGAT tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   TGAT(C242S,C253S) (TRIO Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C242S, C253S

   Promoter

   CMV

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNAD1

   Plasmid

   #154347

   Purpose

   pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

   Depositor

   John van der Oost

   Article

   Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

   Insert

   ShBle
   

   Use

   Microalgae

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Violaxanthin Chlorophyll a Binding Protein Precur…

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Use

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Raichu-OsRac1 WT

   Plasmid

   #133262

   Purpose

   Biosensor for monitoring activation for rice small GTPase OsRac1

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1 (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

   Plasmid

   #154229

   Purpose

   Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

   Plasmid

   #138417

   Purpose

   CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Inserts

   BirA
   dCas9
   

   Use

   Lentiviral

   Tags

   FLAG, Avi-tag and V5, His

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_Ef1a:PE2-P2A-hMLH1dn

   Plasmid

   #184444

   Purpose

   Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLENTI6.3/AR-GC-E2325

    Plasmid

    #85128

    Purpose

    exogenous AR expression

    Depositor

    Karl-Henning Kalland

    Article

    Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

    Insert

    androgen receptor (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ELF3_pet28a

    Plasmid

    #131658

    Purpose

    Bacterial expression of ETS gene ELF3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF3 (ELF3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-C2-SNAP

    Plasmid

    #208794

    Purpose

    For AAV production to express C2-SNAP in astrocytes

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Use

    AAV

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPCX-Cx43-IRES-GFP

    Plasmid

    #65433

    Purpose

    Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge