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Plasmid#200674PurposeModular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
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pLJM1-Empty
Plasmid#91980PurposeEmpty backboneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Rab5a-7
Plasmid#55322PurposeLocalization: Ras GTPase, Excitation: 399, Emission: 456DepositorInsertRab5a
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-tdMCP-24xGCN4
Plasmid#205157Purposeexpress tdMCP-24xGCN4DepositorInserttdMCP-24xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Plasmid#173872PurposeMammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)DepositorInsertmCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.1
Plasmid#139478PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH47751
Plasmid#48002DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE3
Plasmid#197147PurposeExpresses ApoE3 in Mammalian cellDepositorInsertApoE3 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGCN2-FLAG-IP
Plasmid#101794PurposeRetroviral vector encoding C-terminally FLAG tagged mouse GCN2DepositorInsertGcn2 (Eif2ak4 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg
Plasmid#60508PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-ERCreER-4x6T
Plasmid#196411PurposeAstrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertERT2-Cre-ERT2
UseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorUseTagsGST and HisExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-B
Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab5CA (Q79L)
Plasmid#35140DepositorInsertRab5a (RAB5A Human)
UseTagsEGFPExpressionMammalianMutationConstitutively Active- Q79LPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
dCas9-hHDAC3
Plasmid#98591Purpose3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.DepositorInsertdCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEgRNA
Plasmid#172716PurposeDelivery of guide RNA for prime editingDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pHCMV_OROV_GP
Plasmid#207297PurposeMammalian expression of Oropouche virus GP proteinDepositorInsertOROV_GP (M Oropouche virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-LEXY (pDN122)
Plasmid#72655PurposeBlue light-inducible nuclear export of mCherryDepositorInsertNLS-mCherry-LEXY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MS2_P65_HSF1_2A_puro
Plasmid#118699PurposeTo express MS2-P65-HSF1DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0078 RESCUE
Plasmid#130661PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/DepositorInsertdRanCas13b-ADAR2dd (RESCUE)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-Golgin84_VSVG-SBP-EGFP
Plasmid#65305Purposesynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_JEV_prME
Plasmid#207283PurposeMammalian expression of Japanese encephalitis virus PrME proteinDepositorInsertJEV_PrME (POLY Japanese encephalitis virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_MOKV_G
Plasmid#207294PurposeMammalian expression of Mokola virus G proteinDepositorInsertMOKV_G (G Mokola virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV V5-LUC Blast (w567-1)
Plasmid#21474Purpose3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, BlastDepositorInsertV5-LUC
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Lb
Plasmid#86197PurposeLbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterMaize ubiquitin 1Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMo130-TelR
Plasmid#50799PurposeSuicide vector for generating chromosomal deletions in multidrug-resistant bacteriaDepositorInserttellurite-resistance cassette
UseSuicide vectorTagsExpressionMutationPromoterPCs12Available sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Giantin_C1
Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-spikeRBD::∆ACE2 SURF
Plasmid#206957PurposeFluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2DepositorUseTagscSURF, mCherry, and nSURFExpressionMammalianMutation17-615 and RBD (319-541)PromoterCMVAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pZW1-snoVector
Plasmid#73174PurposeNuclear expression of RNAsDepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_HTNV_GP
Plasmid#207281PurposeMammalian expression of Hantaan virus GP proteinDepositorInsertHTNV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723
Plasmid#48015PurposePlease see depositor comments section.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
HypoxCR
Plasmid#59946PurposeA lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.DepositorInsertsGFP-Pest
mCherry-Geminin
UseLentiviralTagsFLAG and PESTExpressionMammalianMutationPromoterCMV and min CMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Plasmid#173871PurposeMammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorInsertmCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only