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pZac2.1 gfaABC1D-BioID2-BioID2-HA

Plasmid

#176740

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSBBi-Pur LaG17-synNotch-TetRVP64

Plasmid

#158133

Purpose

Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

Depositor

Neal Devaraj

Insert

LaG17-synNotch-TetRVP64

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6-1 and dU6-3

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-TC

Plasmid

#183754

Purpose

AAVS1 targeting construct with the Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox257

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2a KI

Plasmid

#131486

Purpose

Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRha-ABE8e-SpCas9-NG

Plasmid

#201190

Purpose

Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

Depositor

Jonathan Yen

Article

Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

Insert

ABE8e-SpCas9-NG

Use

Tags

8xHIS and BP-NLS

Expression

Bacterial

Mutation

Promoter

pRhaBAD

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3A

Plasmid

#35521

Purpose

Mammalian expression of human DNMT3A with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3A (DNMT3A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3 N-MYC TIP60

Plasmid

#213003

Purpose

expresses human TIP60

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

KAT5 (KAT5 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Use

Tags

Expression

Mammalian

Mutation

T130A L131S

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only