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pCMV6-AN-DDK_S580A POLI

Plasmid

#131238

Purpose

Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA POLI

Plasmid

#131239

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert lysine resi…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LRRFIP2

Plasmid

#21152

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

LRRFIP2

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2009

Availability

Academic Institutions and Nonprofits only
RCAS(B) dnFREK (CT#1037)

Plasmid

#13901

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

FREK

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pUCDMp10

Plasmid

#170461

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LTRi

Plasmid

#15913

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TLD_027

Plasmid

#19697

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Insert

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
TLD_062

Plasmid

#19698

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
TLD_026

Plasmid

#19693

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT062a

Plasmid

#107909

Purpose

W2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

UBC-TetR-P2A-LacI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pASCV-HhN-Ren

Plasmid

#37676

Depositor

Philip Beachy

Article

Yao et al Cell. 2006 Apr 21;125(2):343-57.

Insert

HhN

Use

Luciferase

Tags

renilla luciferase

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pPac0

Plasmid

#12094

Depositor

Robert Tjian

Article

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
IELMV

Plasmid

#24596

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Feb 16. ():.

Insert

IE-LM

Use

Tags

Venus

Expression

Mutation

Promoter

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-IDH2-R172K

Plasmid

#87927

Purpose

Expresses IDH2 (R172K mutant) in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

Insert

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)

Use

Tags

none

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-Sox2-3'UTR

Plasmid

#63895

Purpose

Luciferase reporter of mouse wildtype Sox2 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
pPGKFLPobpA

Plasmid

#13793

Purpose

Expression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.

Depositor

Philippe Soriano

Article

Raymond et al PLoS ONE. 2007 . 2():e162.

Insert

FLPo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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