Addgene

Showing: 1681 - 1700 of 18442 results

Results per page:

Sort results by:

pAAV-CAGGS-Flex/5'USS-GFP

Plasmid

#197884

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Has Service

AAV1

Insert

hM4D-2a-GFP (CHRM4 Human, Synthetic)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p264 pSP72 hAR-LBD(LNCaP)

Plasmid

#12495

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Use

Tags

Expression

Bacterial

Mutation

Thr 878 Ala in AR-LBD

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
MSCV P2Gm p53

Plasmid

#19751

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP300-pAAV-CMV-MCS2-pA

Plasmid

#112138

Purpose

CMV driven Multi Cloning Site-2

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SL016: AAV-CAG-FLEX-CheRiff-GFP

Plasmid

#124776

Purpose

For AAV based expression of CheRiff in Cre-Positive cells

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

CheRiff-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mutation

Promoter

CAG

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only
pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_K700E_hSF3B1

Plasmid

#176591

Purpose

Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP306-pAAV-EFS-MCS3-pA

Plasmid

#113682

Purpose

EFS driven Multi Cloning Site-3.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS4 (Empty insertion vector with SEC)

Plasmid

#154837

Purpose

Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

SEC

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

sqt-1(e1350)

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-VSFP2.3 rev. with Flex

Plasmid

#87948

Purpose

Plasmid used (after linearization) to generate transgenic mice that express the genetically encoded voltage indicator upon Cre recombination.

Depositor

Thomas Knopfel

Article

Quinn et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Voltage-Sensitive Fluorescent Protein 2.3

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552-DIO-NLS-mRuby3(with gRNA scaffold)

Plasmid

#199583

Purpose

Expresses NLS-mRuby3 in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

NLS-mRuby3

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1a

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

Plasmid

#216276

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Expression

Mutation

Promoter

human synapsin 1 (hSyn)

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-Flex-Rev-TdTomato

Plasmid

#122501

Purpose

Cre dependent TdTomato expression.

Depositor

Kimberly Ritola

Insert

TdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge