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pYPQ146-ZmUbi-tRNA

Plasmid

#158404

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

Plasmid

#169332

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ePPEmax*

Plasmid

#205244

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Tags

Expression

Plant

Mutation

Detailed in manuscript

Promoter

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

Plasmid

#169327

Purpose

Expression of CEP41-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag7 (CEP41 Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-tRNA

Plasmid

#158402

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Use

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:GrRenilla

Plasmid

#118067

Purpose

Transcriptional unit for constitutive expression of GrRenilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Tags

Expression

Mutation

Promoter

U6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xPP7_SL]

Plasmid

#68424

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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p5xNF-kb_RE

Plasmid

#118050

Purpose

GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

5 copies of the NF-kb DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE3-4

Plasmid

#219946

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:FLuc

Plasmid

#118063

Purpose

Transcriptional unit for constitutive expression of FLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:FLuc:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry

Plasmid

#210603

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

truncated hsp70

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCdkn2a/Cre

Plasmid

#89644

Purpose

Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Cdkn2a (Cdkn2a Mouse)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Use

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKeap1/Cre

Plasmid

#89645

Purpose

Expresses a Keap1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Keap1 (Keap1 Mouse)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-Syn21-V5-mCherry-P10

Plasmid

#104617

Purpose

Expresses V5 tagged mCherry upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-V5-mCherry-P10-MhcSA

Use

Tags

V5

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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