Showing: 1661 - 1680 of 3192 results
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Plasmid#203556PurposeMammalian expression of GST tagged ATG13 (402-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (402-450)
Plasmid#203557PurposeMammalian expression of GST tagged ATG13 (402-450)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (450-517)
Plasmid#203558PurposeMammalian expression of GST tagged ATG13 (450-517)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (6-196)
Plasmid#203559PurposeMammalian expression of GST tagged ATG13 (6-196)DepositorInsertATG13 (ATG13 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-msfGFP-ATG13 (1-197)
Plasmid#203560PurposeMammalian expression of GFP tagged ATG13 (1-197)DepositorInsertATG13 (ATG13 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-MBP
Plasmid#203561PurposeMammalian expression of ATG13 (363-517) with MBP tagDepositorInsertATG13 (ATG13 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-ULK1 (836-1050)-MBP
Plasmid#203562PurposeMammalian expression of ULK1 (836-1050) with MBP tagDepositorInsertULK1 (ULK1 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H2BC11
Plasmid#207755PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBA1B
Plasmid#207763PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-TUBB4B
Plasmid#207785PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pJAT31
Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1AvailabilityAcademic Institutions and Nonprofits only -
pDSK519-cspB
Plasmid#207162PurposeExpresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vectorDepositorInsertcspB
UseTagsHAExpressionBacterialMutationPromoternptIIAvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-msEEA1 HDR template
Plasmid#214990PurposeEndogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (Eea1 Mouse)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1
Plasmid#214994PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215326PurposeLentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertdNTP63 Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterdNTP63AvailabilityAcademic Institutions and Nonprofits only -
pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215327PurposeLentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertMUC5AC Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterMUC5ACAvailabilityAcademic Institutions and Nonprofits only
Showing: 1661 - 1680 of 3192 results