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Plasmid#160069PurposeConditionally overexpress collybistin (CB2SH3-) and mCitrineDepositorInsertARHGEF9 (Arhgef9 Rat)
UseAAVTagsHA- in CB2SH3ExpressionMutationPromoterhuman Synapsin promoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I
Plasmid#130223PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-D[C333G]
UseSynthetic BiologyTagsExpressionMutationC333GPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I
Plasmid#130225PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-F[C336A]
UseSynthetic BiologyTagsExpressionMutationC336APromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-018 IRES-A[G338A](H-I)//Esp3I
Plasmid#130220PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-A[G338A]
UseSynthetic BiologyTagsExpressionMutationG338APromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-019 IRES-B[C335T](H-I)//Esp3I
Plasmid#130221PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-B[C335T]
UseSynthetic BiologyTagsExpressionMutationC335TPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-020 IRES-C[A334G](H-I)//Esp3I
Plasmid#130222PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-C[A334G]
UseSynthetic BiologyTagsExpressionMutationA334GPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-019 FLAG IFP1.4[1] IRES IFP1.4[2]
Plasmid#130129PurposePPI (BiFC/TriFC) assay vectorDepositorInsertIFP1.4
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-020 FLAG miRFP670[1] IRES miRFP670[2]
Plasmid#130130PurposePPI (BiFC/TriFC) assay vectorDepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-022 IRES-E[T337A](H-I)//Esp3I
Plasmid#130224PurposeEMMA Kit # 1000000119 extensionDepositorInsertIRES-E[T337A]
UseSynthetic BiologyTagsExpressionMutationT337APromoterAvailable sinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS
Plasmid#131354PurposeExpresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimusDepositorInsertCaspase-1 (C285A)-NpLxIS (Casp1 Mouse)
UseRetroviralTagsExpressionMammalianMutationCaspase-1 (C285A)PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA
Plasmid#121748PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporterDepositorInsertOLLAS epitope tag-IRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/T35A-IRES-Puro
Plasmid#120571PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, T35APromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/Y40A-IRES-Puro
Plasmid#120573PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, Y40APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-NRASQ61K/E37A-IRES-Puro
Plasmid#120572PurposeExpresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationQ61K, E37APromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147L-siResist
Plasmid#49856PurposeEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100, Methionine 125, and Methionine 147…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49222PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49231PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-GGG/ACC-mEGFP-IRES-mCherry
Plasmid#49230PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49220PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MXW-PGK-c-let-7-LP4B-IRES-EGFP
Plasmid#37647DepositorInsertcel-let-7
UseRetroviralTagsExpressionMammalianMutationLP4 - AATATTACCA=>AATAAAACCAPromotermPGKAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only