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Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest-623 (attR4-attR3)
Plasmid#161878PurposeMultisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR188
Plasmid#79543PurposeOutput plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-Notch1 3'UTR
Plasmid#84595PurposeRenilla luciferase reporter containing NOTCH1 3' UTRDepositorInsertNOTCH1 3'UTR (NOTCH1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 Q67L
Plasmid#107105PurposeLentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationQ67LPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP
Plasmid#75452PurposePlasmid for constitutive expression of gfpDepositorInsertgfp
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_TEAD1_1
Plasmid#86356PurposeEncodes gRNA for 3' target of human TEAD1DepositorInsertgRNA against TEAD1 (TEAD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
c-Myc-5-HT2A
Plasmid#67944PurposeN-terminally c-myc tagged human 5-hydroxytryptamine receptor 2ADepositorInsertHTR2A (HTR2A Human)
UseTagsc-MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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R777-E151 Hs.PAK3
Plasmid#70435PurposeGateway ORF clone of human PAK3 [NM_002578.3] with stop codon (for native or N-terminal fusions)DepositorInsertPAK3 (PAK3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.iAChSnFR-Venus-NULL
Plasmid#137960PurposeExpresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoterDepositorArticleInsertiAChSnFR (non-binding yellow variant)
UseAAV and Cre/LoxTagsExpressionMammalianMutationY140A binding site mutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-TRE-EGFP
Plasmid#89875PurposeEGFP reporter vector of iTango system for AAV productionDepositorInserttetO-EGFP
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-Q61L-shortlinker
Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
gag-TadCBEd
Plasmid#225144PurposePackaging plasmid for TadCBEd in v3 PE-eVLP architectureDepositorInsertMMLVgag-TadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mito-mEmerald
Plasmid#174542Purposelabels mitochondria with mEmeraldDepositorInsertMTS-mEmerald
UseLentiviralTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-mCherry-CAAX
Plasmid#218189PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MSX1
Plasmid#222557PurposePiggyBac transposon plasmid for doxycycline inducible expression of MSX1DepositorInsertMSX1 (MSX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SV2A HaloTag
Plasmid#218545PurposeLentiviral expression of SV2A HaloTagDepositorInsertSV2A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SaCas9-BlastR
Plasmid#220966PurposeExpresses human codon-optimized SaCas9DepositorInsertSaCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI SV2A pHluorin
Plasmid#218543PurposeMamallian Expression of SV2A pHluorinDepositorInsertSV2A
UseTagsExpressionMammalianMutationS42F, L199PPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRT-Fc FL
Plasmid#221853PurposeExpress human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.DepositorInsertcalreticulin (CALR Human)
UseTagsHuman IgG1Fc tagExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE FLAG-IKK2-EE puro
Plasmid#216790PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterLTRAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Dlx-FLEX-hM4Di-mCherry
Plasmid#209701PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent mannerDepositorInserthM4D(Gi)-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_AAMP:1-434
Plasmid#211192PurposeMammalian expression of full-length human AAMP. N-terminal HiBiT tag.DepositorInsertAAMP:M1-R434 (AAMP Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GluN3A/NR3A glutamate receptor [N416/57R]
Plasmid#206618PurposeMammalian Expression Plasmid of anti-GluN3A/NR3A glutamate receptor (Mouse). Derived from hybridoma N416/57.DepositorInsertanti-GluN3A/NR3A glutamate receptor (Mus musculus) recombinant Mouse monoclonal antibody (Grin3a Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
UseTagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pD649-HAsp-CD276-COMP5AP-AviTag-9xHis
Plasmid#157425PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCD276 (CD276 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
Plasmid#176396PurposeExpresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.DepositorInsertGAL1 promoter, SZ1-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_Puro_ccdb_Ubc_rtTA
Plasmid#172130PurposeDestination vector for AAVS1 targetingDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterPGK1Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBP-GFP-Tac
Plasmid#162505PurposeExpresses GFP tagged Tac in a RUSH vector in mammalian cellsDepositorInsertTac (IL2RA Human)
UseTagsGFP and SBPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GRP94-COMP-blac-flag-his
Plasmid#111008PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertendoplasmin, putative (GRP94) (PFL1070c Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-mTagBFP2-T2A-iCreERT2
Plasmid#177308PurposeCAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposonDepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_EGFP_KCNQ1
Plasmid#173161PurposeFor mammalian expression of KCNQ1 and creating variantsDepositorInsertKCNQ1 (KCNQ1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-alphaTubulin_C1
Plasmid#189768PurposeConstruct for labelling microtubules in mammalian cells with mScarlet3-alphatubulinDepositorInsertmScarlet3-alphaTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-FLAG-cGAS
Plasmid#127661PurposeOver-express EGFP-FLAG-cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsEGFP-FlagExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only