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Plasmid#119129PurposeActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.DepositorInsertmCherry and eGFP
UseLentiviralTagsExpressionMutationeGFP L202SPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0047-CMV-dPspCas13b-ADAR1DD(E1008Q)
Plasmid#103863PurposedPspCas13b-ADAR1DD(E1008Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNADepositorInsertsUseCRISPRTagsExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags3XFLAG-Cas9ExpressionMammalianMutationPromoterCBh; U6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Vps34-Flag
Plasmid#24398DepositorInsertVps34 (PIK3C3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2241
Plasmid#141985PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA3 (GATA3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300
Plasmid#191764PurposeExpression of EGFP fused to core p300 histone acetyltransferaseDepositorInsertFRB-EGFP-p300 core (EP300 Human)
UseTagsEGFP (N-terminal of p300 core) and FRB (N-termina…ExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
V48 ELP
Plasmid#68395PurposeElastin-like polypeptide (VPGxG) with 48 repeats of valine guest residueDepositorInsertV48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-mito
Plasmid#168304PurposeChemogenetic fluorescent H2O2 reporter targeted to the mitochondriaDepositorInsertHyPer7.2DAAO-mito
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TPI in pCW57_tTA_Blast
Plasmid#154209PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPIDepositorInsertTPI1 (TPI1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TagBFP-hCoilin
Plasmid#182589Purposeexpresses bfp-hcoilin in mammalian cellsDepositorInsertCoilin (COIL Human)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLX-PGK-hCDK4
Plasmid#114314PurposeConstitutive expression of the CDK4 proteinDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT128c
Plasmid#138512PurposeP2DepositorInsertinteinC-dSpCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mOrange-pBAD
Plasmid#54751PurposeLocalization: Protein Expression Vector , Excitation: 548, Emission: 562DepositorTypeEmpty backboneUseTags6xHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRB895
Plasmid#124586PurposeC-terminal fusions of C. albicans optimized mNeonGreen fluorescent protein.DepositorTypeEmpty backboneUseSynthetic Biology; Candida albicans expressionTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
gag-COM-pol
Plasmid#211373PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-COM-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B-Rigor
Plasmid#172204PurposeExpresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorInsertMS2-P65-HSF1 (HSF1 Human, Synthetic)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3B
Plasmid#137757PurposeExpression of LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro
Plasmid#213030PurposeExpresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)
Plasmid#145839PurposeLentiviral vector genome to ectopically express human Ace2DepositorInserthuman Ace2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB542
Plasmid#74876PurposeGateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-2xFYVE
Plasmid#136996PurposeLentiviral EGFP lipid sensor for PI(3)PDepositorInsertEGFP-2xFYVE
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB541
Plasmid#74875PurposeGateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
IL-2 promoter luciferase
Plasmid#12194DepositorInsertIL-2 promoter (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-L
Plasmid#59025PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-L
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-SNAP
Plasmid#119949PurposeLentiviral vector expressing G3BP1-SNAP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsSNAPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDriver
Plasmid#165149PurposeDriver plasmid for PROBER encoding SV40 Large T-antigenDepositorInsertSV40 Large T-antigen
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV2-TBG-FLAG-mRIPK1
Plasmid#115341PurposeLiver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1DepositorInsertRIPK1 (Ripk1 Mouse)
UseAAVTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJL4
Plasmid#199379PurposeEncodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEr
Plasmid#61208PurposeFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-mCherry-BCP
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1
Plasmid#194279PurposeExpresses gRNA and VP64-dSaCas9-VP64 from lentiviral vectorDepositorInsertshumanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only