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C1C2CMUNC2C

Plasmid

#218674

Purpose

Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

MUNC13 (Unc13a Rat)

Use

Tags

GST

Expression

Insect

Mutation

Amino acids1407-1453 deleted

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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CpX-1

Plasmid

#218676

Purpose

Expresses complexin-1 in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

Rat complexin-1 (Cplx1 Rat)

Use

Tags

6 his

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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vGlut1 peptide- GST

Plasmid

#218677

Purpose

Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

vGlut (Slc17a6 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat PGK1 shRNA

Plasmid

#222869

Purpose

Expresses mTagBFP2 along with an shRNA against rat PGK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1 shRNA (Pgk1, Pgk1 Rat, Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T160C

Plasmid

#220233

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T160C

Promoter

T7

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(85-160)

Plasmid

#217984

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-100)

Plasmid

#217978

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T2C, C90A, T198C

Plasmid

#220235

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T2C, C90A, T198C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C, T259C

Plasmid

#220234

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C, T259C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6GB1-CREB(1-280) C90A

Plasmid

#217982

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) A50C,C90A

Plasmid

#217985

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

A50C, C90A

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280)

Plasmid

#217981

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(160-280)

Plasmid

#217980

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Article

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218867

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218866

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF1A tail

Plasmid

#218800

Purpose

Mammalian expression of rat Kif 1A

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

Kif 1A (Kif1a Rat)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-YTH-EGFP

Plasmid

#209322

Purpose

AAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTH-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-YTHmut-EGFP

Plasmid

#209323

Purpose

AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1--YTHmut-HA

Use

AAV

Tags

HA

Expression

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1-YTH

Plasmid

#209319

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTH-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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