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pcDNA3-myc3-CUL4A

Plasmid

#19951

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-TVA-eGFP-N2cG

Plasmid

#175439

Purpose

Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDV10_pCMV-ADAR1-p150

Plasmid

#200405

Purpose

Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p150

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEF_PE2

Plasmid

#199267

Purpose

Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Use

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-N EBNA1

Plasmid

#37954

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV flag ERR alpha

Plasmid

#10975

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

ERR alpha (ESRRA Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only

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lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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