Addgene

We narrowed to 13,070 results for: ung

Showing: 1641 - 1660 of 13070 results

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TALEN1259

Plasmid

#32284

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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TALEN1258

Plasmid

#32281

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1/Pur

Plasmid

#171682

Purpose

constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

OsTIR1-MYC

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3B-CDS

Plasmid

#136045

Purpose

UPF3B shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GAAGCCTTGTTCCGATCTAAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3B (UPF3B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMEH361 Hspa8 MS2 HA

Plasmid

#168218

Purpose

Mammalian expression of heat shock 70 kDa protein 8 fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Hspa8

Use

Tags

HA and MS2 dimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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rNrx1beta(47-299)

Plasmid

#20173

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neurexin 1 beta (Nrxn1 Rat)

Use

Tags

6X His tag

Expression

Insect

Mutation

no splice site

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
PLKO.1-UPF2-CDS

Plasmid

#136040

Purpose

UPF2 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCGTTATGTTTGGTGGAAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Article

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pCY 3090-07

Plasmid

#36232

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-Zeocin

Expression

Mutation

Promoter

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pJH2930

Plasmid

#175283

Purpose

Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Pges-1

Available since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiN 16E6no*

Plasmid

#37445

Purpose

Lentiviral expression of HPV 16E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 16E6no* (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

V42L

Promoter

CMV

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK3-ZC3H15

Plasmid

#136015

Purpose

ZC3H15 3'UTR (NM_018471.2) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

ZC3H15 3'UTR (ZC3H15 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

Plasmid

#52478

Purpose

Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJBL713

Plasmid

#140383

Purpose

Expresses CtcS protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CtcS-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Article

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Use

Tags

Expression

Yeast

Mutation

Promoter

PMA1

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Use

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
PLKO.1-UPF2-3'UTR

Plasmid

#136041

Purpose

UPF2 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CGCGAGGGTTAATCTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GFP Snail 2SA

Plasmid

#16226

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

2SA: S97A, S101A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only