-
Plasmid#164196PurposeTo express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansionDepositorInsertMf BulKRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationY269A;Y347FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimEL(TA)
Plasmid#23093DepositorInsertBim EL (TA) (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Threonine 112 to AlaninePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-rat ERK2-D319N
Plasmid#40820DepositorInsertMAPK1 (Mapk1 Rat)
UseTagsHis6ExpressionMammalianMutationD319NPromoterCMVAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 P191G, P192A
Plasmid#12213DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPIX (PAK-interacting exchange factor) binding mut…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimEL(TD)
Plasmid#23092DepositorInsertBim EL (TD) (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Threonine 112 to AspartatePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP G116A
Plasmid#123098PurposeExpresses 3xFLAG-GABARAP G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R-UGI
Plasmid#161817PurposeExpresses ABE-P48R-UGI in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(APY)
Plasmid#15742DepositorInsertJnk1a1(TPF) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationThr-190AlaPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-NG-nCas9
Plasmid#163551PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 HA (#1709)
Plasmid#14594DepositorInsertLexA
UseTagsHA, Ubiquitin, and VP16ExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(TPF)
Plasmid#15741DepositorInsertJnk1a1(APY) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationTyr-192PhePromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV ShadowG-AURKA-mTurq2
Plasmid#157768PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and shadowGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag p38 alpha
Plasmid#20786DepositorInsertpCMV5 Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
p270 pCMV-CRE-M-AR(LNCaP)
Plasmid#12496DepositorInsertCRE-M AR (AR Human)
UseCre/LoxTagsExpressionMammalianMutationThr 868 Ala in AR-LBDPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3A G120
Plasmid#123091PurposeExpresses 3xFLAG-LC3A G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 121. Stop codon after G120.PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV mTurquoise2-shadowY tandem
Plasmid#157775PurposeProduces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneUseTagsmTurquoise2 and shadowYExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS61APromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Entry IL36RN S113L
Plasmid#58321Purposeentry vector for human IL36RN S113L mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationS113L: c.338C>T, p.Ser113LeuPromoterCMVAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-del 50-59
Plasmid#33065DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 50-59PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-deltaY20
Plasmid#58459PurposeExpresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsmycExpressionMammalianMutationTyrosine 20 deletedPromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only