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Plasmid#153281PurposeEnd-linker for cloning 3 sgRNA arrays into any recipient vectorDepositorInsertlinker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE510
Plasmid#153282PurposeEnd-linker for cloning 4 sgRNA arrays into any recipient vectorDepositorInsertlinker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE506
Plasmid#153279PurposeIntermediate sgRNA array cloning vector position 4DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE505
Plasmid#153278PurposeIntermediate sgRNA array cloning vector position 3DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMNK55 - piRNAi 21ur-1224 - cku-80
Plasmid#159820PurposepiRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombinationDepositorInsertcku-80 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_47
Plasmid#60071PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-5
Plasmid#73856PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-4
Plasmid#73855PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fluorescent reporter for MOBE3-4
Plasmid#219946PurposeMammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4DepositorInsertCMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
UseCRISPRTagsExpressionMammalianMutationEGFP(A111V/L202S)PromoterCMV, U6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PV1_VP1
Plasmid#202071PurposeBacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertPV1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL_SV40:PuroR
Plasmid#183039PurposeExpresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL_SV40:PuroR
Plasmid#183041PurposeExpresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL_SV40:PuroR
Plasmid#183042PurposeExpresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only