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Plasmid#133505PurposeExpression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0816
Plasmid#133510PurposeExpression of mTagBFP2 tagged CRIB domain to monitor active Cdc42DepositorInsertpAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
ARB349
Plasmid#124032PurposeEncodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFPDepositorInsertL2-C3_CAG-Lyn::tagBFP-SV40_RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
ARB341
Plasmid#124024PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFPDepositorInsertLS-A4_EF1a-NLS::tagBFP-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB345
Plasmid#124028PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFPDepositorInsertL1-B1_CMV-NES::tagBFP-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0455
Plasmid#124040PurposeEncodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-NES-tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_009
Plasmid#123956PurposeEncodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertT2A::NLS::EBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_039
Plasmid#123914PurposeEncodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_014
Plasmid#123868PurposeEncodes WPRE expressing tagBFP T2A Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-tagBFP-T2A-Blast (no terminator)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_004
Plasmid#123858PurposeEncodes tagBFP, thosea asigna virus 2A (T2A) and Blasticidin resistance as a multicistronic cassette to be used in the MTK systemDepositorInsertCMV-tagBFP-T2A-BLAST-bgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_016
Plasmid#123829PurposeEncodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertP2a-NLS::tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_017
Plasmid#123830PurposeEncodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertP2a-NLS::EBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_022
Plasmid#123774PurposeEncodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK systemDepositorInserthis::GAI::tagBFP::linker
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_023
Plasmid#123775PurposeEncodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK systemDepositorInserthis::ABI::tagBFP::linker
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Cytbow
Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT007
Plasmid#171634PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-TRAC
mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMALB
Plasmid#161786PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsTagBFPExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only